Visual Protein成立于2005年，致力于通過(guò)為研究提供創(chuàng)新產(chǎn)品和服務(wù)來(lái)加強(qiáng)生物開(kāi)發(fā)。我們的優(yōu)勢(shì)是我們的研發(fā)能力。Visual Protein開(kāi)發(fā)的大多數(shù)產(chǎn)品比公司開(kāi)發(fā)的產(chǎn)品更有效。我們的專(zhuān)業(yè)知識(shí)拓寬了蛋白質(zhì)組學(xué)，免疫學(xué)和細(xì)胞學(xué)領(lǐng)域的研究可能性。
VisualProtein BC03-500說(shuō)明書(shū)
Dual-Range™BCA蛋白質(zhì)分析試劑盒是常用的蛋白質(zhì)分析方法。它基于二辛可寧酸（BCA），用于比色檢測(cè)和定量5-2,000μg/ mL濃度范圍內(nèi)的總蛋白。與Lowry方法一樣，該分析依賴(lài)于蛋白質(zhì)對(duì)Cu 2+離子的還原。由此形成的Cu +通過(guò)與二辛可寧酸反應(yīng)轉(zhuǎn)化成紫色物質(zhì)來(lái)檢測(cè)。Dual-Range™BCA蛋白質(zhì)分析試劑盒與許多洗滌劑兼容，但與DTT，DTE和2-巰基乙醇等還原劑不相容。
BCA蛋白質(zhì)測(cè)定基于堿性溶液中蛋白質(zhì)將Cu 2+還原為Cu +，通過(guò)二辛可寧酸（BCA）對(duì)Cu +進(jìn)行靈敏且特異性的比色檢測(cè)。兩個(gè)BCA分子與每個(gè)Cu +螯合，使還原的銅從蘋(píng)果綠色變?yōu)樽仙珡?fù)合物，在562nm處具有強(qiáng)吸收。
| 貨號(hào) | 產(chǎn)品名稱(chēng) | 描述 |
BC03-500 |
Dual-Range™BCA蛋白質(zhì)分析試劑盒 |
1個(gè)套件 500mL試劑A + 12 mL試劑 B + 10mL BSA（2mg / mL）
用于制作2500個(gè)微孔板測(cè)定
|
圖1.通過(guò)使用微孔板程序（37℃，30分鐘）的牛血清白蛋白（BSA）和牛γ球蛋白（BGG）的標(biāo)準(zhǔn)曲線。
圖2.通過(guò)使用微孔板程序（37℃，30分鐘），用5％Triton X-100或8％SDS的BSA的標(biāo)準(zhǔn)曲線。
圖3.通過(guò)使用
具有增強(qiáng)方案的微孔板程序（60℃持續(xù)30分鐘）使用微孔板程序的BSA的標(biāo)準(zhǔn)曲線。
| 貨號(hào) | 品名 | 規(guī)格 |
| BC03-500 | Dual-RangeTM BCA protein assay kit | 1 kit |
| BR05-500 | Bradford Reagent (5X) | 1 kit |
| BR05-500-K | Bradford Protein Assay Kit (5X) | 1 kit |
| BP01-1L | BlockPROTM Blocking Buffer | 1 kit |
| BF01-1L | BlockPROTM Protein-Free Blocking Buffer | 1 kit |
| BF10-100 | BlockPROTM Protein-Free Blocking Buffer (10X) | 1 kit |
| BF20-50P | BlockPROTM Protein-Free Blocking Buffer (20X) | 1 kit |
| BM01-500 | BlockPRO 1 Min Protein-Free Blocking Buffer | 1 kit |
| BM10-100 | BlockPRO 1 Min Protein-Free Blocking Buffer (10X) | 1 kit |
| BOX12 | Western Blot Tool Box | 1 kit |
| BOX12-03 | Western Blot Tool Box | 1 kit |
| EP05-30 | ExtractPROTM Protein Extraction Reagent (Phenol included) | 1 bottle |
| FP01-5 | FracPROTM Protein Fractionation Kit | 1 kit |
| FP01-50 | FracPROTM Protein Fractionation Kit | 1 kit |
| HB01-1L | HybriMoreTM Hybridoma Cloning Factor | 1 bottle |
| IF01-4N | ImmunoFastTM Adjuvant | 1 kit |
| IF01-20N | ImmunoFastTM Adjuvant | 1 kit |
| LF01-10S | LumiFlashTM Prime Chemiluminescent Substrate, HRP (sample size) | 1 kit |
| LF01-100 | LumiFlashTM Prime Chemiluminescent Substrate, HRP | 1 kit |
| LF01-500 | LumiFlashTM Prime Chemiluminescent Substrate, HRP | 1 kit |
| LF08-10S | LumiFlashTM Ultima Chemiluminescent Substrate, HRP (sample size) | 1 kit |
| LF08-100 | LumiFlashTM Ultima Chemiluminescent Substrate,HRP | 1 kit |
| LF08-500 | LumiFlashTM Ultima Chemiluminescent Substrate,HRP | 1 kit |
| LF16-10S | LumiFlashTM Infinity Chemiluminescent Substrate,HRP (sample size) | 1 kit |
| LF16-100 | LumiFlashTM Infinity Chemiluminescent Substrate, HRP | 1 kit |
| LF16-500 | LumiFlashTM Infinity Chemiluminescent Substrate, HRP | 1 kit |
| LH03-10 | LuminolPenTM, HRP System | 1 pen |
| LH03-50 | LuminolPenTM, HRP System | 1 pen |
| PBS10-1L | PBS Buffer, 10X (pH 7.4) | 1 kit |
| PBST10-1L | PBS Buffer, 10X (pH 7.4), with 0.5% Tween 20 | 1 kit |
| PP03-2C | PhosPROTM Phosphoprotein Enrichment Kit | 1 kit |
| PP03-5E | PhosPROTM phosphoprotein Enrichment Kit | 1 kit |
| PP03-6C | PhosPROTM Phosphoprotein Enrichment Kit | 1 kit |
| PP05-2C | PhosPROTM Phosphoprotein Purification Kit | 1 kit |
| PP05-5E | PhosPROTM Phosphoprotein Purification Kit | 1 kit |
| PP05-6C | PhosPROTM Phosphoprotein Purification Kit | 1 kit |
| RB10-1L | SDS-PAGE Running Buffer (Tris-Glycine, pH 8.3), 10X | 1 kit |
| RB500P | SDS-PAGE Running Buffer (Tris-Glycine), powder pack | 1 kit |
| RP05-10 | 5X RIPA Cell Lysis Buffer (sample size) | 1 bottle |
| RP05-100 | 5X RIPA Cell Lysis Buffer | 1 bottle |
| RP05-500 | 5X RIPA Cell Lysis Buffer | 1 bottle |
| SBN06-15 | 6x Laemmli SDS Sample Buffer, non-reducing | 1 kit |
| SBR06-15 | 6x Laemmli SDS Sample Buffer, reducing | 1 kit |
| SP05-100 | StripPRO 1 Min Stripping Buffer (5X) | 1 kit |
| TBS10-1L | TBS Buffer, 10X (pH 7.4) | 1 kit |
| TBST10-1L | TBS Buffer, 10X (pH 7.4), with 0.5% Tween 20 | 1 kit |
| TBST200P | TBS Buffer, 25X (pH7.4), with 0.5% Tween 20 | 1 kit |
| VC01-25S | VisColorTM Pre-Stained Protein Marker | 1 vial |
| VC01-250 | VisColorTM Pre-Stained Protein Marker | 1 vial |
| VC01-500 | VisColorTM Pre-Stained Protein Marker | 2 vials |
| VC03-25S | VisColorTM Full-Range Pre-Stained Protein Marker | 1 vial |
| VC03-250 | VisColorTM Full-Range Pre-Stained Protein Marker | 1 vial |
| VC03-500 | VisColorTM Full-Range Pre-Stained Protein Marker | 2 vials |
| VP01-125 | VisPROTM 5 Minutes Protein Stain Kit (sample size) | 1 kit |
| VP01-500 | VisPROTM 5 Minutes Protein Stain Kit | 1 kit |
| VP05-125 | 5X VisPROTM 5 Minutes Protein Stain Kit (sample size) | 1 kit |
| VP05-500 | 5X VisPROTM 5 Minutes Protein Stain Kit | 1 kit |
| WTB10-1L | Western Blot Transfer Buffer (Tris-Glycine, pH 8.3), 10X | 1 kit |
| WTB500P | Western Blot Transfer Buffer, powder pack | 1 kit |
| BSB01 | Black Staining Box (for VisPRO Protein Stain Kit) | 1 box |
| BSB02 | Black Staining Box (for VisPRO Protein Stain Kit) | 1 box |
| BSB03 | Black Staining Box (for VisPRO Protein Stain Kit) | 1 box |
| BSB04 | Black Staining Box (for VisPRO Protein Stain Kit) | 1box |
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